Characterization and Quantitation of Concanavalin A Binding by Plasma Membrane Enriched Fractions from Soybean Root
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چکیده
منابع مشابه
Characterization and quantitation of concanavalin a binding by plasma membrane enriched fractions from soybean root.
The binding of concanavalin A (Con A) to soybean root membranes in plasma membrane enriched fractions (recovered from the 34/45% interface of simplified discontinuous sucrose density gradients) was studied using a radiochemical assay employing tritiated ((3)H)-Con A. The effect of lectin concentration, time, and membrane protein concentration on the specific binding of (3)H-Con A by the membran...
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The polypeptide complement of enriched soybean (Glycine max [L.] Merr. cult. wells) root plasma membrane fractions was studied by two-dimensional gel electrophoresis. Good resolution was obtained when polypeptides were solubilized in sodium dodecyl sulfate and when butylated hydroxytoluene was included in the vesicle isolation and solubilization media. The pattern obtained on the two-dimensiona...
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Plasma membrane-enriched fractions were obtained from the Balb/c 3T12 cell line by a conventional technique. The specific activities of two plasma membrane markers, 5’-nucleotidase and (Na+-K+)-ATPase were enriched loto l&fold and 6to lo-fold, respectively, over homogenates in these preparations. Galactosyltransferase activity toward N-acetylglucosamine and asialo-agalacto-fetuin was associated...
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Methods have been developed for the isolation on a semi-micro scale of a plasma membrane-enriched fraction from rat islets of Langerhans. An important feature of these experiments is the use of 125I-labeled wheat germ agglutinin as a specific probe for plasma membrane-containing fractions. The partly purified plasma membrane fraction had a density in sucrose of about 1.10 and was enriched in th...
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Rat intestinal Golgi-enriched membrane fractions take up Ca2+ by a vitamin D-dependent process that has been shown to recover within 15 min of repletion of vitamin D-deficient animals with intravenous 1,25-dihydroxycholecalciferol. The present paper reports studies characterizing the Ca2+-binding sites of these membrane fractions. Equilibrium binding of Ca2+ at concentrations between 5 and 400 ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1981
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.68.5.1014